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library(netassoc)
set.seed(1)
# Number of m species
nsp <- 10
# Number of n sites
nsi <- 50
# Observed m x n community matrix (abundance or presence/absence)
m_obs <- floor(matrix(rpois(nsp*nsi,lambda=5),ncol=nsi,nrow=nsp))
# "Force" some species associations to the observed community matrix
m_obs[1,1:(nsi/2)] <- rpois(n=nsi/2,lambda=20)
m_obs[2,1:(nsi/2)] <- rpois(n=nsi/2,lambda=20)
# Null expected m x n community matrix (abundance or presence/absence)
m_nul <- floor(matrix(rpois(nsp*nsi,lambda=5),ncol=nsi,nrow=nsp))
Note that here we are simply resampling the observed data preserving row and column sums, which is NOT recommended. Instead, we should use our expected null model of community assembly.
# What species co-occurrence patterns are more or less likely than expected under the null model?
n <- make_netassoc_network(m_obs, m_nul,
method="partial_correlation",
args=list(method="shrinkage"), # for alternative estimators see ?partial_correlation
p.method='fdr',
numnulls=100,
plot=TRUE,
alpha=0.05)
## Calculating observed co-occurrence scores...
## Generating null replicate 1...
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## Calculating standardized effect sizes...
## Adjusting p-values for multiple comparisons...
## Building network...
n$network_all
## IGRAPH DNW- 10 2 --
## + attr: name (v/c), weight (e/n)
## + edges (vertex names):
## [1] Species 1->Species 2 Species 2->Species 1